A Novel On‐Chip Method for Differential Extraction of Sperm in Forensic Cases

Yazarlar

F. Inci, M. Ozen, Yeşeren Saylan, Morteza Miansari, Duygu Çimen, Raghu Dhara, T. Chinnasamy, Mehmet Yuksekkaya, C. Filippini, D. Kumar, S. Çalamak, Yusuf Yesil, N. G. Durmus, G. Duncan, L. Klevan, U. Demirci

Yayın

Advancement of science 2018

DOI

10.1002/advs.201800121

Atıflar

46

Özet

One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time‐consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60–90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl‐LewisX), a major carbohydrate ligand for sperm‐egg binding. This method is validated with forensic mock samples dating back to 2003, resulting in 70–92% sperm capture efficiency and a 60–92% reduction in epithelial fraction. Captured sperm are then lysed on‐chip and sperm DNA is isolated. This method reduces assay‐time from 8 h to 80 min, providing an inexpensive alternative to current differential extraction techniques, accelerating identification of suspects and advancing public safety.

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BibTeX

@article{f2018,
  title   = {A Novel On‐Chip Method for Differential Extraction of Sperm in Forensic Cases},
  author  = {F. Inci and M. Ozen and Yeşeren Saylan and Morteza Miansari and Duygu Çimen and Raghu Dhara and T. Chinnasamy and Mehmet Yuksekkaya and C. Filippini and D. Kumar and S. Çalamak and Yusuf Yesil and N. G. Durmus and G. Duncan and L. Klevan and U. Demirci},
  journal = {Advancement of science},
  doi     = {10.1002/advs.201800121},
  year    = {2018}
}

← Tüm yayınlar DOI: 10.1002/advs.201800121