Antibody purification from human plasma by metal‐chelated affinity membranes

Yazarlar

H. Yavuz, Nilay Bereli̇, C. Armutçu, Fatma Yılmaz, A. Deni̇zli̇

Yayın

2012

DOI

10.1002/APP.34672

Atıflar

9

Özet

The aim of this study is to investigate in detail the feasibility of poly(2‐hydroxyethyl methacrylate‐N‐methacryloyl‐(L)‐histidine methyl ester), PHEMAH membranes for purification of immunoglobulin G (IgG) from human plasma. PHEMAH membranes were prepared by photo‐polymerization technique. Then, Zn2+, Ni2+, Co2+, and Cu2+ ions were chelated directly on the PHEMAH membranes. Elemental analysis assay was performed to determine the nitrogen content and polymerized MAH was calculated as 168.5 μmol/g. The nonspecific IgG adsorption onto the plain PHEMA membranes was negligible (about 0.25 mg/mL). A remarkable increase in the IgG adsorption capacities were achieved from human plasma with PHEMAH membranes (up to 68.4 mg/mL). Further increase was observed with the metal‐chelated PHEMAH membranes (up to 118 mg/mL). The metal‐chelate affinity membranes allowed the one‐step separation of IgG from human plasma. The binding range of metal ions for surface histidines from human plasma followed the order: Cu2+ > Ni2+ > Zn2+ > Co2+. Adsorbed IgG was eluted using 250 mM EDTA with a purity of 94.1%. IgG molecules could be repeatedly adsorbed and eluted with the metal‐chelated PHEMAH membranes without noticeable loss in their IgG adsorption capacity. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012

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BibTeX

@article{h2012,
  title   = {Antibody purification from human plasma by metal‐chelated affinity membranes},
  author  = {H. Yavuz and Nilay Bereli̇ and C. Armutçu and Fatma Yılmaz and A. Deni̇zli̇},
  doi     = {10.1002/APP.34672},
  year    = {2012}
}

← Tüm yayınlar DOI: 10.1002/APP.34672