Dopamine‐imprinted monolithic column for capillary electrochromatography

Yazarlar

S. Aşır, D. Sarı, Ali Derazshamshir, Fatma Yılmaz, Koray Şarkaya, A. Deni̇zli̇

Yayın

Electrophoresis 2017

DOI

10.1002/elps.201700228

Atıflar

19

Özet

A dopamine‐imprinted monolithic column was prepared and used in capillary electrochromatography as stationary phase for the first time. Dopamine was selectively separated from aqueous solution containing the competitor molecule norepinephrine, which is similar in size and shape to the template molecule. Morphology of the dopamine‐imprinted column was observed by scanning electron microscopy. The influence of the organic solvent content of mobile phase, applied pressure and pH of the mobile phase on the recognition of dopamine by the imprinted monolithic column has been evaluated, and the imprinting effect in the dopamine‐imprinted monolithic polymer was verified. Developed dopamine‐imprinted monolithic column resulted in excellent separation of dopamine from structurally related competitor molecule, norepinephrine. Separation was achieved in a short period of 10 min, with the electrophoretic mobility of 5.81 × 10–5 m2V–1s–1 at pH 5.0 and 500 mbar pressure.

İlgili araştırmalar

BiyosensörlerBiyokromatografi

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BibTeX

@article{s2017,
  title   = {Dopamine‐imprinted monolithic column for capillary electrochromatography},
  author  = {S. Aşır and D. Sarı and Ali Derazshamshir and Fatma Yılmaz and Koray Şarkaya and A. Deni̇zli̇},
  journal = {Electrophoresis},
  doi     = {10.1002/elps.201700228},
  year    = {2017}
}

← Tüm yayınlar DOI: 10.1002/elps.201700228