Hemoglobin Purification Using His-Tag Affinity Chromatography With the Assistance of Ni Ions
Muhammed Erkek, Merve Çalışır, A. A. Topçu, A. Deni̇zli̇
- Yazarlar
- Muhammed Erkek, Merve Çalışır, A. A. Topçu, A. Deni̇zli̇
- Yayın
- Hacettepe Journal of Biology and Chemistry 2024
- DOI
- 10.15671/hjbc.1454485
- Atıflar
- 0
Özet
In this study, an affinity adsorbent of poly(2-hydroxyethyl methacrylate) [(p(HEMA)] was developed to purify hemoglobin (Hb). Initially, cryogel membranes of p(HEMA) were prepared and their surfaces were modified with the dopamine monomer to create polydopamine (pda) modified p(HEMA) cryogel [pda(HEMA)] membranes. After the modification, the nickel (Ni) ions were immobilized on pda(HEMA ) for Hb purification in the aqueous solution. At pH 5, 23.5 mg/g Hb was adsorbed by the adsorbent and the Langmuir adsorption isotherm model was suitable for this adsorption process thanks to the R2 and q max values; moreover, the prepared adsorbent without notable decreases in its adsorption capacity
Bu makaleye atıf yapın
Muhammed Erkek, Merve Çalışır, A. A. Topçu, A. Deni̇zli̇ (2024). Hemoglobin Purification Using His-Tag Affinity Chromatography With the Assistance of Ni Ions. Hacettepe Journal of Biology and Chemistry. https://doi.org/10.15671/hjbc.1454485
BibTeX
@article{muhammed2024,
title = {Hemoglobin Purification Using His-Tag Affinity Chromatography With the Assistance of Ni Ions},
author = {Muhammed Erkek and Merve Çalışır and A. A. Topçu and A. Deni̇zli̇},
journal = {Hacettepe Journal of Biology and Chemistry},
doi = {10.15671/hjbc.1454485},
year = {2024}
}