Molecularly imprinted based surface plasmon resonance nanosensors for microalbumin detection

Yazarlar

Meltem Koca Esentürk, Semra Akgönüllü, Fatma Yılmaz, A. Deni̇zli̇

Yayın

Journal of Biomaterials Science. Polymer Edition 2019

DOI

10.1080/09205063.2019.1600181

Atıflar

35

Özet

Human serum albumin (HSA) is a major blood plasma protein also found in urine where its existence may be a marker of some types of liver or kidney dysfunction. Herein, we fabricated a novel surface plasmon resonance (SPR) nanosensor for selective, sensitive, and label-free microalbumin detection both in aqueous and urine sample solutions. First, HSA-imprinted nanoparticles were synthesized, which consist of ethylene glycol dimethacrylate and N-methacryloyl-L-leucine methyl ester as a cross-linker and functional monomer. The nanoparticles were characterized by zeta-size and scanning electron microscope analyses and were dropped onto the SPR chip surface to make HSA sensitive nanosensor. Characterization studies of HSA-imprinted SPR chip were carried out by atomic force microscopy, Fourier-transform infrared spectroscopy, contact angle, and ellipsometer. The limit of detection and limit of quantification values of HSA-imprinted SPR nanosensor were calculated as 0.7 pM and 1.9 pM for the concentration range of 0.15–500 nM. Selectivity studies of HSA-imprinted SPR nanosensor were achieved with hemoglobin and transferrin proteins which were chosen as competitor molecules. HSA-imprinted SPR nanosensor was displayed highly selective and sensitive to HSA.

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BibTeX

@article{meltem2019,
  title   = {Molecularly imprinted based surface plasmon resonance nanosensors for microalbumin detection},
  author  = {Meltem Koca Esentürk and Semra Akgönüllü and Fatma Yılmaz and A. Deni̇zli̇},
  journal = {Journal of Biomaterials Science. Polymer Edition},
  doi     = {10.1080/09205063.2019.1600181},
  year    = {2019}
}

← Tüm yayınlar DOI: 10.1080/09205063.2019.1600181