Molecularly Imprinted Nanofilms for Endotoxin Detection Using an Surface Plasmon Resonance Sensor.

Yazarlar

Duygu Çimen, Sevgi Aslıyüce, T. D. Tanalp, A. Deni̇zli̇

Yayın

Analytical Biochemistry 2021

DOI

10.1016/j.ab.2021.114221

Atıflar

24

Özet

In this study, a simple, fast, sensitive and selective surface plasmon resonance (SPR) sensor has been prepared using molecular imprinting method for endotoxin detection. Endotoxin imprinted and non-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) based polymeric films were synthesized onto SPR chip surfaces using ultraviolet polymerization. Endotoxin imprinted and non-imprinted SPR sensors were characterized by using contact angle, atomic force microscopy and ellipsometry. After characterization studies, kinetic studies were carried out in the concentration range of 0.5-100 ng/mL. The limit of detection and quantification were obtained as 0.023 and 0.078 ng/mL, respectively. The response time for the equilibration, adsorption and regeneration was approximately 14 min. The selectivity studies with cholesterol and hemoglobin of SPR sensor were examined. Validation studies were carried out via limulus amebocyte lysate (LAL) in order to demonstrate the applicability of the SPR sensor.

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BibTeX

@article{duygu2021,
  title   = {Molecularly Imprinted Nanofilms for Endotoxin Detection Using an Surface Plasmon Resonance Sensor.},
  author  = {Duygu Çimen and Sevgi Aslıyüce and T. D. Tanalp and A. Deni̇zli̇},
  journal = {Analytical Biochemistry},
  doi     = {10.1016/j.ab.2021.114221},
  year    = {2021}
}

← Tüm yayınlar DOI: 10.1016/j.ab.2021.114221