Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM) for Bilirubin Detection

Yazarlar

Çiğdem Çiçek, Fatma Yılmaz, Erdoğan Özgür, H. Yavuz, A. Deni̇zli̇

Yayın

2016

DOI

10.3390/CHEMOSENSORS4040021

Atıflar

38

Özet

This study aims the preparation of a QCM sensor for the detection of bilirubin in human plasma. Bilirubin-imprinted poly-(2-hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester) (PHEMATrp) nanofilm (MIP) on the gold surface of a QCM chip was synthesized by the molecular imprinting technique. Meanwhile, the non-imprinted PHEMATrp (NIP) nanofilm was synthesized by the same experimental technique to examine the imprinting effect. Characterization of MIP and NIP nanofilms on the QCM chip surface was achieved by atomic force microscopy (AFM), ellipsometry, Fourier transform infrared spectrophotometry-attenuated total reflectance (FTIR-ATR) and contact angle measurements (CA). The observations indicated that the nanofilm was almost in a monolayer. Thereinafter, the imprinted and the non-imprinted QCM chips were connected to the QCM system to investigate kinetic and affinity properties. In order to examine the selectivity of the MIP-PHEMATrp nanofilm, competitive adsorption of bilirubin with cholesterol and estradiol was performed. Limit of detection (LOD) and limit of quantitation (LOQ) values were calculated as 0.45 μg/mL and 0.9 μg/mL, respectively.

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BibTeX

@article{idem2016,
  title   = {Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM) for Bilirubin Detection},
  author  = {Çiğdem Çiçek and Fatma Yılmaz and Erdoğan Özgür and H. Yavuz and A. Deni̇zli̇},
  doi     = {10.3390/CHEMOSENSORS4040021},
  year    = {2016}
}

← Tüm yayınlar DOI: 10.3390/CHEMOSENSORS4040021