Optical Based Microcontact Imprinted Sensor for Detection of Egg Ovalbumin

Yazarlar

Z. Kurt, Duygu Çimen, A. Deni̇zli̇, Nilay Bereli̇

Yayın

IEEE Sensors Journal 2026

DOI

10.1109/JSEN.2026.3675700

Atıflar

0

Özet

In this study, ovalbumin imprinted surface plasmon resonance (SPR) sensor was developed for the sensitive and selective detection of ovalbumin. The surfaces of ovalbumin imprinted and nonimprinted (NIP) poly(hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester) SPR sensors were characterized by atomic force microscopy (AFM), contact angle measurements and Fourier-transform infrared spectroscopy. Kinetic analyses were performed in phosphate buffer solution (pH 7.4) with ovalbumin concentrations ranging from 0.005 to 1.5 ng/mL. The detection limit in ovalbumin solutions was determined to be 0.00019 ng/mL. Selectivity studies demonstrated that the developed sensor exhibited higher affinity toward ovalbumin compared with lysozyme and transferrin. The relative selectivity coefficients of the ovalbumin-imprinted SPR sensor for ovalbumin/lysozyme and ovalbumin/transferrin were 5.94 and 8.05, respectively. This study also demonstrates that a high-molecular-weight protein such as ovalbumin can be successfully imprinted using the microcontact imprinting technique, providing a significant advancement in protein imprinting strategies. Therefore, the proposed SPR sensor offers a highly sensitive, selective, and reliable platform for the rapid detection of food allergens in complex matrices.

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BibTeX

@article{z2026,
  title   = {Optical Based Microcontact Imprinted Sensor for Detection of Egg Ovalbumin},
  author  = {Z. Kurt and Duygu Çimen and A. Deni̇zli̇ and Nilay Bereli̇},
  journal = {IEEE Sensors Journal},
  doi     = {10.1109/JSEN.2026.3675700},
  year    = {2026}
}

← Tüm yayınlar DOI: 10.1109/JSEN.2026.3675700