Polymethacryloyl-L-Phenylalanine [PMAPA]-Based Monolithic Column for Capillary Electrochromatography.

Yazarlar

Ali Derazshamshir, S. Aşır, Ilgım Göktürk, Sisem Ektirici, Fatma Yılmaz, A. Deni̇zli̇

Yayın

Journal of Chromatographic Science 2019

DOI

10.1093/chromsci/bmz047

Atıflar

6

Özet

The ability to detect catecholamines (CAs) and their metabolites is vital to understand the mechanism behind the neuronal diseases. Neurochemistry aims to provide an improved pharmacological, molecular and physiological understanding of complex brain chemistries by analytical techniques. Capillary electrophoresis (CE) is one such analytical technique that enables the study of various chemical species ranging from amino acids and peptides to natural products and drugs. CE can easily adapt the changes in research focus and in recent years remains an applicable technique for investigating neuroscience and single cell neurobiology. The prepared phenylalanine-based hydrophobic monolithic column, Polymethacryloyl-L-phenylalanine [PMAPA], was used as a stationary phase in capillary electrochromatography to separate CAs that are similar in size and shape to each other including dopamine (DA) and norepinephrine (NE) via hydrophobic interactions. Separation carried out in a short period of 17 min was performed with the electrophoretic mobility of 5.54 × 10-6 m2 V-1 s-1 and 7.60 × 10-6 m2 V-1 s-1 for DA and NE, respectively, at pH 7.0, 65% acetonitrile ratio with 100 mbar applied pressure by the developed hydrophobic monolithic column without needing any extra process such as imprinting or spacer arms to immobilize ligands used in separation.

İlgili araştırmalar

Biyokromatografi

Bu makaleye atıf yapın

BibTeX

@article{ali2019,
  title   = {Polymethacryloyl-L-Phenylalanine [PMAPA]-Based Monolithic Column for Capillary Electrochromatography.},
  author  = {Ali Derazshamshir and S. Aşır and Ilgım Göktürk and Sisem Ektirici and Fatma Yılmaz and A. Deni̇zli̇},
  journal = {Journal of Chromatographic Science},
  doi     = {10.1093/chromsci/bmz047},
  year    = {2019}
}

← Tüm yayınlar DOI: 10.1093/chromsci/bmz047