Preparation of cryogel columns for depletion of hemoglobin from human blood
Ali Derazshamshir, G. Baydemir, Fatma Yılmaz, Nilay Bereli̇, A. Deni̇zli̇
- Yazarlar
- Ali Derazshamshir, G. Baydemir, Fatma Yılmaz, Nilay Bereli̇, A. Deni̇zli̇
- Yayın
- Artificial Cells Nanomedicine and Biotechnology 2016
- DOI
- 10.3109/21691401.2015.1129623
- Atıflar
- 10
Özet
In this study, we aimed to prepare the metal chelate affinity cryogels for the hemoglobin (Hb) depletion. Poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were selected as base matrix because of their blood compatibility, osmotic, chemical, and mechanical stability. Cryogels are also useful when working with the viscous samples such as blood, because of their interconnected macroporous structure. Iminodiacetic acid (IDA), the chelating agent, was covalently coupled with PHEMA cryogels after activation with the epichlorohydrin and then the Ni(II) ions were chelated to the IDA-bound cryogels. The depletion of the Hb from hemolysate was shown by SDS-PAGE.
İlgili araştırmalar
Bu makaleye atıf yapın
Ali Derazshamshir, G. Baydemir, Fatma Yılmaz, Nilay Bereli̇, A. Deni̇zli̇ (2016). Preparation of cryogel columns for depletion of hemoglobin from human blood. Artificial Cells Nanomedicine and Biotechnology. https://doi.org/10.3109/21691401.2015.1129623
BibTeX
@article{ali2016,
title = {Preparation of cryogel columns for depletion of hemoglobin from human blood},
author = {Ali Derazshamshir and G. Baydemir and Fatma Yılmaz and Nilay Bereli̇ and A. Deni̇zli̇},
journal = {Artificial Cells Nanomedicine and Biotechnology},
doi = {10.3109/21691401.2015.1129623},
year = {2016}
}