Protein A-immobilized microporous polyhydroxyethylmethacrylate affinity membranes for selective sorption of human-immunoglobulin-G from human plasma
A. Deni̇zli̇, Y. Arıca
- Yazarlar
- A. Deni̇zli̇, Y. Arıca
- Yayın
- Journal of Biomaterials Science. Polymer Edition 2000
- DOI
- 10.1163/156856200743760
- Atıflar
- 28
Özet
Microporous membranes made of poly(2-hydroxyethylmethacrylate) [poly(HEMA)] carrying protein A were used for selective sorption of human-IgG from human plasma. Poly(HEMA) membranes were prepared by a photo-polymerization technique, and activated by cyanogen bromide (CNBr) in an alkaline medium (pH 11.5). Bioligand protein A was then immobilized by covalent binding onto these CNBr-activated membranes. The amount of immobilized protein A was controlled by changing pH and the initial concentrations of CNBr and protein A. The non-specific adsorption of protein A on the plain poly(HEMA) membranes was 2.9 microg cm(-2). Maximum protein A immobilization was observed at pH 9.5. Up to 186 microg cm(-2) was immobilized on the CNBr-activated poly(HEMA) membranes. The maximum adsorption of human-IgG on the protein A-immobilized poly(HEMA) membranes was observed at pH 8.0. The non-specific adsorption of human-IgG onto the plain poly(HEMA) membranes was low (about 4.4 microg cm(-2)). Higher human-IgG adsorption values (up to 394 microg cm(-2)) were obtained in which the protein A-immobilized poly(HEMA) membranes were used. Much higher amounts of human-IgG (up to 489 microg cm(-2)) were adsorbed from human plasma. Up to 91% of the adsorbed human-IgG was desorbed by using 0.1 M aminoacetic acid as elution agent. The adsorption-desorption cycle was repeated ten times using the same polymeric membranes. There was no remarkable reduction in the adsorption capacity of the protein A-immobilized poly(HEMA) membranes.
Bu makaleye atıf yapın
A. Deni̇zli̇, Y. Arıca (2000). Protein A-immobilized microporous polyhydroxyethylmethacrylate affinity membranes for selective sorption of human-immunoglobulin-G from human plasma. Journal of Biomaterials Science. Polymer Edition. https://doi.org/10.1163/156856200743760
BibTeX
@article{a2000,
title = {Protein A-immobilized microporous polyhydroxyethylmethacrylate affinity membranes for selective sorption of human-immunoglobulin-G from human plasma},
author = {A. Deni̇zli̇ and Y. Arıca},
journal = {Journal of Biomaterials Science. Polymer Edition},
doi = {10.1163/156856200743760},
year = {2000}
}