Selective Amplification of Plasmonic Sensor Signal for Cortisol Detection Using Gold Nanoparticles

Yazarlar

Gaye Ezgi Yılmaz, Yeşeren Saylan, Ilgım Göktürk, Fatma Yılmaz, A. Deni̇zli̇

Yayın

Biosensors 2022

DOI

10.3390/bios12070482

Atıflar

34

Özet

Herein, gold nanoparticles (AuNP)-modified cortisol-imprinted (AuNP-MIP) plasmonic sensor was developed for signal amplification and real-time cortisol determination in both aqueous and complex solutions. Firstly, the sensor surfaces were modified with 3-(trimethoxylyl)propyl methacrylate and then pre-complex was prepared using the functional monomer N-methacryloyl-L-histidine methyl ester. The monomer solution was made ready for polymerization by adding 2-hydroxyethyl methacrylate to ethylene glycol dimethacrylate. In order to confirm the signal enhancing effect of AuNP, only cortisol-imprinted (MIP) plasmonic sensor was prepared without AuNP. To determine the selectivity efficiency of the imprinting process, the non-imprinted (AuNP-NIP) plasmonic sensor was also prepared without cortisol. The characterization studies of the sensors were performed with atomic force microscopy and contact angle measurements. The kinetic analysis of the AuNP-MIP plasmonic sensor exhibited a high correlation coefficient (R2 = 0.97) for a wide range (0.01–100 ppb) with a low detection limit (0.0087 ppb) for cortisol detection. Moreover, the high imprinting efficiency (k′ = 9.67) of the AuNP-MIP plasmonic sensor was determined by comparison with the AuNP-NIP plasmonic sensor. All kinetic results were validated and confirmed by HPLC.

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BibTeX

@article{gaye2022,
  title   = {Selective Amplification of Plasmonic Sensor Signal for Cortisol Detection Using Gold Nanoparticles},
  author  = {Gaye Ezgi Yılmaz and Yeşeren Saylan and Ilgım Göktürk and Fatma Yılmaz and A. Deni̇zli̇},
  journal = {Biosensors},
  doi     = {10.3390/bios12070482},
  year    = {2022}
}

← Tüm yayınlar DOI: 10.3390/bios12070482