Sensitive and real-time detection of IgG using interferometric reflecting imaging sensor system.
Monireh Bakhshpour, Elisa Chiodi, Iris Celebi, Yeşeren Saylan, N. Ünlü, M. Ünlü, A. Deni̇zli̇
- Yazarlar
- Monireh Bakhshpour, Elisa Chiodi, Iris Celebi, Yeşeren Saylan, N. Ünlü, M. Ünlü, A. Deni̇zli̇
- Yayın
- Biosensors & bioelectronics 2022
- DOI
- 10.1016/j.bios.2021.113961
- Atıflar
- 16
Özet
Considering the limitations of well-known traditional detection techniques, innovative research studies have focused on the development of new sensors to offer label-free, highly sensitive, real-time, low-cost, and rapid detection for biomolecular interactions. In this study, we demonstrate immunoglobulin G (IgG) detection in aqueous solutions by using real-time and label-free kinetic measurements of the Interferometric Reflectance Imaging Sensor (IRIS) system. By performing kinetic characterization experiments, the sensor's performance is comprehensively evaluated and a high correlation coefficient value (>0.94) is obtained in the IgG concentration range of 1-50 μg/mL with a low detection limit (0.25 μg/mL or 1.67 nM). Moreover, the highly sensitive imaging system ensures accurate quantification and reliable validation of recorded binding events, offering new perspectives in terms of direct biomarker detection for clinical applications.
İlgili araştırmalar
Bu makaleye atıf yapın
Monireh Bakhshpour, Elisa Chiodi, Iris Celebi, Yeşeren Saylan, N. Ünlü, M. Ünlü et al. (2022). Sensitive and real-time detection of IgG using interferometric reflecting imaging sensor system.. Biosensors & bioelectronics. https://doi.org/10.1016/j.bios.2021.113961
BibTeX
@article{monireh2022,
title = {Sensitive and real-time detection of IgG using interferometric reflecting imaging sensor system.},
author = {Monireh Bakhshpour and Elisa Chiodi and Iris Celebi and Yeşeren Saylan and N. Ünlü and M. Ünlü and A. Deni̇zli̇},
journal = {Biosensors & bioelectronics},
doi = {10.1016/j.bios.2021.113961},
year = {2022}
}