Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for l-Phenylalanine Detection
Duygu Çimen, Nilay Bereli̇, A. Deni̇zli̇
- Yazarlar
- Duygu Çimen, Nilay Bereli̇, A. Deni̇zli̇
- Yayın
- Biosensors 2021
- DOI
- 10.3390/bios11010021
- Atıflar
- 35
Özet
In this study, we designed a simple, rapid, sensitive and selective surface plasmon resonance (SPR) sensor for detection of L-phenylalaine by utilizing molecular imprinting technology. l-phenylalanine imprinted and non-imprinted poly(2-hydroxyethyl methacrylate-methacryloyl-l-phenylalanine) polymeric films were synthesized onto SPR chip surfaces using ultraviolet polymerization. l-phenyalanine imprinted and non-imprinted SPR sensors were characterized by using contact angle, atomic force microscopy and ellipsometry. After characterization studies, kinetic studies were carried out in the concentration range of 5.0–400.0 μM. The limit of detection and quantification were obtained as 0.0085 and 0.0285 μM, respectively. The response time for the test including equilibration, adsorption and desorption was approximately 9 min. The selectivity studies of the l-phenylalanine imprinted SPR sensor was performed in the presence of d-phenylalanine and l-tryptophan. Validation studies were carried out via enzyme-linked immunosorbent analysis technique in order to demonstrate the applicability and superiority of the l-phenylalanine imprinted SPR sensor.
Bu makaleye atıf yapın
Duygu Çimen, Nilay Bereli̇, A. Deni̇zli̇ (2021). Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for l-Phenylalanine Detection. Biosensors. https://doi.org/10.3390/bios11010021
BibTeX
@article{duygu2021,
title = {Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for l-Phenylalanine Detection},
author = {Duygu Çimen and Nilay Bereli̇ and A. Deni̇zli̇},
journal = {Biosensors},
doi = {10.3390/bios11010021},
year = {2021}
}