Catalase purification from rat liver with iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-glutamic acid) cryogel discs

Yazarlar

Ilgım Göktürk, Işık Perçin, A. Deni̇zli̇

Yayın

Preparative Biochemistry & Biotechnology 2016

DOI

10.1080/10826068.2015.1085400

Atıflar

14

Özet

In this study, iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-glutamic acid) (PHEMAGA/Fe(3+)) cryogel discs were prepared. The PHEMAGA/Fe(3+) cryogel discs were characterized by elemental analysis, scanning electron microscopy, Fourier transform infrared spectroscopy, swelling tests, and surface area measurements. The PHEMAGA/Fe(3+) cryogel discs had large pores ranging from 10 to 100 µm with a swelling degree of 9.36 g H2O/g cryogel. Effects of pH, temperature, initial catalase concentration, and flow rate on adsorption capacity of the PHEMAGA/Fe(3+) cryogel discs were investigated. Maximum catalase adsorption capacity (62.6 mg/g) was obtained at pH 7.0, 25°C, and 3 mg/ml initial catalase concentration. The PHEMAGA/Fe(3+) cryogel discs were also tested for the purification of catalase from rat liver. After tissue homogenization, purification of catalase was performed using the PHEMAGA/Fe(3+) cryogel discs and catalase was obtained with a yield of 54.34 and 16.67 purification fold.

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BibTeX

@article{ilgm2016,
  title   = {Catalase purification from rat liver with iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-glutamic acid) cryogel discs},
  author  = {Ilgım Göktürk and Işık Perçin and A. Deni̇zli̇},
  journal = {Preparative Biochemistry & Biotechnology},
  doi     = {10.1080/10826068.2015.1085400},
  year    = {2016}
}

← Tüm yayınlar DOI: 10.1080/10826068.2015.1085400