Catalase purification from rat liver with iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-glutamic acid) cryogel discs
Ilgım Göktürk, Işık Perçin, A. Deni̇zli̇
- Authors
- Ilgım Göktürk, Işık Perçin, A. Deni̇zli̇
- Published
- Preparative Biochemistry & Biotechnology 2016
- DOI
- 10.1080/10826068.2015.1085400
- Citations
- 14
Abstract
In this study, iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-glutamic acid) (PHEMAGA/Fe(3+)) cryogel discs were prepared. The PHEMAGA/Fe(3+) cryogel discs were characterized by elemental analysis, scanning electron microscopy, Fourier transform infrared spectroscopy, swelling tests, and surface area measurements. The PHEMAGA/Fe(3+) cryogel discs had large pores ranging from 10 to 100 µm with a swelling degree of 9.36 g H2O/g cryogel. Effects of pH, temperature, initial catalase concentration, and flow rate on adsorption capacity of the PHEMAGA/Fe(3+) cryogel discs were investigated. Maximum catalase adsorption capacity (62.6 mg/g) was obtained at pH 7.0, 25°C, and 3 mg/ml initial catalase concentration. The PHEMAGA/Fe(3+) cryogel discs were also tested for the purification of catalase from rat liver. After tissue homogenization, purification of catalase was performed using the PHEMAGA/Fe(3+) cryogel discs and catalase was obtained with a yield of 54.34 and 16.67 purification fold.
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Cite this article
Ilgım Göktürk, Işık Perçin, A. Deni̇zli̇ (2016). Catalase purification from rat liver with iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-glutamic acid) cryogel discs. Preparative Biochemistry & Biotechnology. https://doi.org/10.1080/10826068.2015.1085400
BibTeX
@article{ilgm2016,
title = {Catalase purification from rat liver with iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-glutamic acid) cryogel discs},
author = {Ilgım Göktürk and Işık Perçin and A. Deni̇zli̇},
journal = {Preparative Biochemistry & Biotechnology},
doi = {10.1080/10826068.2015.1085400},
year = {2016}
}