Overview
Chromatographic separation techniques for purification and analysis of biological molecules and pharmaceuticals.
Our biochromatography research focuses on developing novel stationary phases and separation methods for the purification of proteins, nucleic acids, and other biomolecules. We emphasize creating materials with high selectivity, capacity, and biocompatibility.
Research Areas
Affinity Chromatography
Development of affinity ligands and supports for highly selective purification based on specific biological interactions.
- Protein A/G mimetic ligands
- Metal chelate affinity chromatography (IMAC)
- Dye-ligand affinity
- Molecularly imprinted affinity materials
- Cryogel affinity columns
Ion Exchange Chromatography
Charged stationary phases for separation based on electrostatic interactions.
- Strong and weak exchangers
- Mixed-mode ion exchangers
- Monolithic ion exchange columns
Size Exclusion Chromatography
Separation based on molecular size for protein characterization and purification.
- Polymer-based SEC materials
- Aggregate analysis
- Molecular weight determination
Hydrophobic Interaction Chromatography
Separation based on hydrophobicity differences between biomolecules.
- Novel HIC ligands
- Protein refolding applications
Current project
Advanced Chromatographic Materials
Development of novel monolithic and particulate materials for high-performance bioseparations.
Key publications
Pseudospecific magnetic affinity beads for immunoglobulin-G depletion from human serum
Sinan Akgöl, Serpil Özkara, L. Uzun, Fatma Yılmaz, A. Deni̇zli̇
2007 · doi:10.1002/APP.26771
Supermacroporous hydrophobic affinity cryogels for protein chromatography
Fatma Yılmaz, Nilay Bereli̇, H. Yavuz, A. Deni̇zli̇
l-Histidine imprinted supermacroporous cryogels for protein recognition
Nilay Bereli̇, Yeşeren Saylan, L. Uzun, R. Say, A. Deni̇zli̇
Antibody purification from human plasma by metal‐chelated affinity membranes
H. Yavuz, Nilay Bereli̇, C. Armutçu, Fatma Yılmaz, A. Deni̇zli̇
2012 · doi:10.1002/APP.34672
Hydrophobic microbeads as an alternative pseudo-affinity adsorbent for recombinant human interferon-α via hydrophobic interactions
Yeşeren Saylan, Müfrettin Murat Sarı, Serpil Özkara, L. Uzun, A. Deni̇zli̇
Cation exchange/hydrophobic interaction monolithic chromatography of small molecules and proteins by nano liquid chromatography.
Cemil Aydoğan, Fatma Yılmaz, A. Deni̇zli̇
Journal of Separation Science 2013 · doi:10.1002/jssc.201300089