Prostate specific antigen (PSA) is an important biomarker for diagnosis and prognosis of prostate can- cer. Herein, microcontact PSA-imprinted surface plasmon resonance (SPR) sensor chip was developed for sensitive, real-time detection of PSA. The imprinted chip was prepared in the presence of methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker via UV poly- merization using microcontact imprinting technique. PSA imprinted SPR sensor chip was characterized by atomic force microscope (AFM), scanning electron microscope (SEM), ellipsometry, dispersive Raman and Fourier transform infrared spectroscopy (FT-IR). Under optimal conditions, PSA detection was per- formed with standard PSA solutions in the concentration range of 0.1–50 ng mL−1 with a detection limit (LOD) of approximately 91 pg mL−1 (18 × 10−14 M). Selectivity studies were performed against human serum albumin (HSA) and lysozyme (Lyz) as the competitive agents. The developed system was evalu- ated for analysis of 10 clinical serum samples and showed approximately 98% agreement between the results obtained by commercial enzyme-linked immunosorbent assay (ELISA) method without significant differences at the 0.05 significance level (p = 0.751, p > 0.05).
