Catalase adsorption onto Cibacron Blue F3GA and Fe(III)-derivatized poly(hydroxyethyl methacrylate) membranes and application to a continuous system
M. Arica, A. Deni̇zli̇, B. Salih, E. Pişkin, V. Hasırcı
- Authors
- M. Arica, A. Deni̇zli̇, B. Salih, E. Pişkin, V. Hasırcı
- Published
- 1997
- DOI
- 10.1016/S0376-7388(96)00334-1
- Citations
- 63
Abstract
Poly(2-hydroxyethyl methacrylate) (poly(HEMA)) membranes were prepared by UV-initiated photopolymerization of HEMA in the presence of an initiator (a-a′-azobisisobutyronitrile, AIBN). An affinity dye, i.e. Cibacron Blue F3GA (CB) was incorporated covalently and then complexed with Fe(III) ions. The polyHEMA-CB and polyHEMA-CB-Fe(III) derivatized membranes were used in the adsorption of catalase (CAT). The enzyme-loading capability of the Fe(III)-containing membrane (23.6 μg/cm2) was greater than that of the poly(HEMA)-CB derivatized membrane (17.1 μg/cm2). The adsorption phenomena appeared to follow a typical Langmuir isotherm. The Km values for both immobilized catalases (poly(HEMA)-CB-CAT (22.4 mM) and poly(HEMA)-CB-Fe(III)-CAT (19.3 mM)) were higher than that of free enzyme (16.5 mM). Optimum operational temperature was 5°C higher than that of the free enzyme and was significantly broader. A similar observation was made for the optimum pH. Operational, thermal and storage stabilities were found to increase with immobilization, especially in the presence of Fe(III). It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss in adsorption capacity or enzyme activity.
Cite this article
M. Arica, A. Deni̇zli̇, B. Salih, E. Pişkin, V. Hasırcı (1997). Catalase adsorption onto Cibacron Blue F3GA and Fe(III)-derivatized poly(hydroxyethyl methacrylate) membranes and application to a continuous system. https://doi.org/10.1016/S0376-7388(96)00334-1
BibTeX
@article{m1997,
title = {Catalase adsorption onto Cibacron Blue F3GA and Fe(III)-derivatized poly(hydroxyethyl methacrylate) membranes and application to a continuous system},
author = {M. Arica and A. Deni̇zli̇ and B. Salih and E. Pişkin and V. Hasırcı},
doi = {10.1016/S0376-7388(96)00334-1},
year = {1997}
}