Collagen and Fibronectin Immobilization on PHEMA Microcarriers for Hepatocyte Attachment

Authors

A. Deni̇zli̇, Erhan Pişkin, Vivek Dixit, M. Arthur, Gary L. Gitnick

Published

International Journal of Artificial Organs 1995

DOI

10.1177/039139889501800208

Citations

25

Abstract

Polyhydroxyethylmethacrylate (PHEMA) microcarriers in a size range of 150-250 microns were prepared by a suspension polymerization in an aqueous phase containing magnesium oxide. The hydroxyl groups on the PHEMA microcarriers were activated by cyanogen bromide. In order to improve cell attachment, cell-adhesive proteins, namely, collagen and fibronectin were immobilized onto PHEMA microcarriers. The nonspecific adsorption values for collagen and fibronectin were 0.10 mg collagen/g PHEMA and 0.044 mg fibronectin/g PHEMA, respectively. Collagen and fibronectin immobilization on PHEMA microcarriers were studied at different pH by using single protein solutions containing different amounts of proteins, at a constant temperature of 20 degrees C. The maximum immobilizations were 0.85 mg collagen/g PHEMA (at pH: 9.5) and 0.52 mg fibronectin/g PHEMA (at pH: 7.4). Hepatocyte attachment onto these biologically modified PHEMA microcarriers was studied. Hydrophilic PHEMA microcarriers did not support cell attachment. High hepatocyte attachment yields (up to 75% surface coverage) were observed on collagen and fibronectin immobilized PHEMA microcarriers.

Cite this article

BibTeX

@article{a1995,
  title   = {Collagen and Fibronectin Immobilization on PHEMA Microcarriers for Hepatocyte Attachment},
  author  = {A. Deni̇zli̇ and Erhan Pişkin and Vivek Dixit and M. Arthur and Gary L. Gitnick},
  journal = {International Journal of Artificial Organs},
  doi     = {10.1177/039139889501800208},
  year    = {1995}
}

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