Development of Molecularly Imprinted Surface Plasmon Resonance Sensor for the Detection of Metrafenone at Maximum Residue Levels
Kıvılcım Çaktü Güler, Ilgım Göktürk, Fatma Yılmaz, F. Kartal, Adil Denizli
- Authors
- Kıvılcım Çaktü Güler, Ilgım Göktürk, Fatma Yılmaz, F. Kartal, Adil Denizli
- Published
- IEEE Sensors Journal 2025
- DOI
- 10.1109/jsen.2025.3574219
- Citations
- 1
Abstract
In this study, metrafenone (MET), a widely used pesticide, was detected in food, soil, and water samples using a molecularly imprinted surface plasmon resonance (MIP@SPR) sensor. For this purpose, MET-imprinted MET imprinted poly [2-hydroxyethyl methacrylate (HEMA)-N-methacryloyl-L-phenylalanine methyl ester (MAPA)] (MET-MIP) nanoparticles were synthesized and immobilized onto the surface of the sensors, thereby creating MET-specific recognition sites. The characterization of MET-MIP nanoparticles was performed using Nano zetasizer measurements and scanning electron microscopy (SEM). The surface characterization of the MIP@SPR sensors was conducted using atomic force microscopy (AFM), contact angle (CA) measurements, and Fourier-transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR) analysis. Kinetic analyses were performed using the SPR system with MET solutions prepared in the concentration range of 0.01–10 mg/L. The obtained results indicated that the limit of detection (LOD) of the MIP@SPR sensor for MET detection was 0.0031 mg/L. For selectivity studies, novaluron was used as the competitor molecule. The MIP@SPR sensors exhibited a 19.92-fold higher selectivity for MET than novaluron. To evaluate the success of the imprinting process, nonimprinted poly [2-hydroxyethyl methacrylate (HEMA)-N-methacryloyl-L-phenylalanine methyl ester (MAPA)] (NIP) nanoparticles were synthesized, and the imprinting factor of the MIP@SPR sensor was calculated as 17.78. For real sample analysis, tomato samples were tested using the MIP@SPR sensor, and high-performance liquid chromatography (HPLC) analysis was conducted to confirm the presence of MET in the samples, thereby validating the results.
Related research
Cite this article
Kıvılcım Çaktü Güler, Ilgım Göktürk, Fatma Yılmaz, F. Kartal, Adil Denizli (2025). Development of Molecularly Imprinted Surface Plasmon Resonance Sensor for the Detection of Metrafenone at Maximum Residue Levels. IEEE Sensors Journal. https://doi.org/10.1109/jsen.2025.3574219
BibTeX
@article{kvlcm2025,
title = {Development of Molecularly Imprinted Surface Plasmon Resonance Sensor for the Detection of Metrafenone at Maximum Residue Levels},
author = {Kıvılcım Çaktü Güler and Ilgım Göktürk and Fatma Yılmaz and F. Kartal and Adil Denizli},
journal = {IEEE Sensors Journal},
doi = {10.1109/jsen.2025.3574219},
year = {2025}
}