- Authors
- H. Yavuz, A. Deni̇zli̇
- Published
- Journal of Biomaterials Science. Polymer Edition 2003
- DOI
- 10.1163/156856203766652020
- Citations
- 16
Abstract
Anti-low density lipoprotein antibody (anti-LDL antibody) attached poly(2-hydroxyethyl methacrylate-methacryloylamidophenylalanine) (poly(HEMA-MAPA)) beads were prepared for selective removal of cholesterol from hypercholesterolemic human plasma. Poly(HEMA-MAPA) beads were produced by a modified suspension polymerization and then characterized by swelling tests and SEM. Blood-compatibility tests were also investigated. The water swelling ratio of the poly(HEMA-MAPA) beads increased significantly (68%) compared with pHEMA (55%). All the clotting times increased when compared with poly(HEMA) beads. Loss of platelets and leukocytes was very low. The maximum anti-LDL antibody attachment was achieved at pH 7.0. Attachment of anti-LDL antibody was 29.6 mg/g. There was a very low non-specific cholesterol binding onto the poly(HEMA-MAPA) beads, about 0.74 mg/g. Anti-LDL antibody attached beads adsorbed in the range of 13.3-16.0 mg cholesterol/g from hypercholesterolemic human plasma. Up to 92% of the adsorbed LDL was desorbed. The binding-elution cycle was repeated 10 times using the same beads. There was no significant loss of binding capacity.
Cite this article
H. Yavuz, A. Deni̇zli̇ (2003). Immunoaffinity beads for selective removal of cholesterol from human plasma. Journal of Biomaterials Science. Polymer Edition. https://doi.org/10.1163/156856203766652020
BibTeX
@article{h2003,
title = {Immunoaffinity beads for selective removal of cholesterol from human plasma},
author = {H. Yavuz and A. Deni̇zli̇},
journal = {Journal of Biomaterials Science. Polymer Edition},
doi = {10.1163/156856203766652020},
year = {2003}
}