Molecularly imprinted cryogel columns for Concanavalin A purification from jack bean extract
Duygu Çimen, Nilay Bereli̇, Müge Andaç, A. Deni̇zli̇
- Authors
- Duygu Çimen, Nilay Bereli̇, Müge Andaç, A. Deni̇zli̇
- Published
- Separation Science Plus 2018
- DOI
- 10.1002/SSCP.201800039
- Citations
- 28
Abstract
Concanavalin A, one of most studied lectins for the purification of glycoproteins and sugars, is selected as a model protein. In this study, a Concanavalin A imprinted poly(hydroxyethyl methacrylate) supermacroporous cryogel was prepared for the purification of Concanavalin from jack bean extract. N ‐Methacryloyl‐ l ‐histidine methyl ester with nickel(II) ions was used as the metal ion coordination complex. Concanavalin A imprinted cryogels were characterized by swelling degree, surface area, Fourier transform infrared spectroscopy, scanning electron microscopy and micro computed tomography measurements. Concanavalin A rebinding and desorption on Concanavalin A imprinted and non‐imprinted cryogels were measured using a continuous system. Selective binding studies were carried out in the presence of Concanavalin B and bovine serum albumin. The selectivity studies were confirmed by fast protein liquid chromatography studies using jack bean extract from Canavalia ensiformis .
Cite this article
Duygu Çimen, Nilay Bereli̇, Müge Andaç, A. Deni̇zli̇ (2018). Molecularly imprinted cryogel columns for Concanavalin A purification from jack bean extract. Separation Science Plus. https://doi.org/10.1002/SSCP.201800039
BibTeX
@article{duygu2018,
title = {Molecularly imprinted cryogel columns for Concanavalin A purification from jack bean extract},
author = {Duygu Çimen and Nilay Bereli̇ and Müge Andaç and A. Deni̇zli̇},
journal = {Separation Science Plus},
doi = {10.1002/SSCP.201800039},
year = {2018}
}