Real time monitoring and label free creatinine detection with artificial receptors
Aykut Arif Topçu, Erdoğan Özgür, Fatma Yılmaz, Nilay Bereli̇, A. Deni̇zli̇
- Authors
- Aykut Arif Topçu, Erdoğan Özgür, Fatma Yılmaz, Nilay Bereli̇, A. Deni̇zli̇
- Published
- Materials Science and Engineering: B 2019
- DOI
- 10.1016/J.MSEB.2019.04.018
- Citations
- 24
Abstract
Molecular imprinting technique is used to design artificial creatinine receptor on the gold surface of surface plasmon resonance (SPR) chip using N-methacryloyl-( l )-histidine methyl ester (MAH), as a functional monomer. Surface characterization of SPR sensor chip is performed with atomic force microscope (AFM), ellipsometry and contact angle measurements. Creatinine imprinted SPR sensor is characterized with a linear range between 1 and 100 mM and limit of detection (LOD) and limit of quantification (LOQ) of creatinine are found 57 μM and 190 μM, respectively. N-hydroxysuccinimide (NHS) and creatine molecules are selected to examine the selectivity of creatinine imprinted SPR sensor. Reusability studies of SPR sensor is determined in urine mimic samples.
Cite this article
Aykut Arif Topçu, Erdoğan Özgür, Fatma Yılmaz, Nilay Bereli̇, A. Deni̇zli̇ (2019). Real time monitoring and label free creatinine detection with artificial receptors. Materials Science and Engineering: B. https://doi.org/10.1016/J.MSEB.2019.04.018
BibTeX
@article{aykut2019,
title = {Real time monitoring and label free creatinine detection with artificial receptors},
author = {Aykut Arif Topçu and Erdoğan Özgür and Fatma Yılmaz and Nilay Bereli̇ and A. Deni̇zli̇},
journal = {Materials Science and Engineering: B},
doi = {10.1016/J.MSEB.2019.04.018},
year = {2019}
}