Selective recognition of nucleosides by boronate affinity organic-inorganic hybrid monolithic column.
Sisem Ektirici, Ilgım Göktürk, Fatma Yılmaz, A. Deni̇zli̇
- Authors
- Sisem Ektirici, Ilgım Göktürk, Fatma Yılmaz, A. Deni̇zli̇
- Published
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2020
- DOI
- 10.1016/j.jchromb.2020.122477
- Citations
- 12
Abstract
Boronic acids are important ligands used to selectively recognize and enrich compounds containing cis-diol groups such as nucleosides. In the present study, organic-inorganic hybrid [POSS-MAH-BPA] monolithic column was prepared for the first time in the literature as a new boronate affinity system for the recognition of nucleosides. The selectivity of the [POSS-MAH-PBA] boronate affinity monolithic column for the recognition of cis-diol containing adenosine nucleoside from its analogue molecule of deoxyadenosine triphosphate, dATP, non-cis-diol containing compound was investigated both by UV and HPLC studies. When the relative selectivity coefficients are compared, the [POSS-MAH-PBA] boronate affinity monolithic column is 4.25 times more selective for adenosine than [POSS-MAH] monolithic column. Besides, to determine endogenous nucleosides in biological fluids, which may serve as non-invasive cancer biomarkers, nucleosides were spiked into the urine solutions and passed through the [POSS-MAH-PBA] boronate affinity monolithic column, and the nucleosides were confirmed by HPLC. The adenosine recognition capability of the [POSS-MAH-PBA] boronate affinity monolithic column with an average enrichment factor of 48.9-fold was apparently superior to that of the [POSS-MAH] monolithic column. Methacryl Polyhedral Oligomeric Silsesquioxanes (POSS-MA) with nano-sized stable 3-dimensional architectures provided the advantage of being used as an adsorbent for the monolithic structure by providing high surface area, 507.60 m2/g, and enabling vinyl groups to function with amino acid-based MAH monomers capable of providing electrons to coordinate PBA. Recovery results of more than 90% for adenosine showed that the [POSS-MAH-PBA] boronate affinity monolithic column could be a promising adsorbent for selective adsorption of cis-diol containing compounds such as nucleosides.
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Cite this article
Sisem Ektirici, Ilgım Göktürk, Fatma Yılmaz, A. Deni̇zli̇ (2020). Selective recognition of nucleosides by boronate affinity organic-inorganic hybrid monolithic column.. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. https://doi.org/10.1016/j.jchromb.2020.122477
BibTeX
@article{sisem2020,
title = {Selective recognition of nucleosides by boronate affinity organic-inorganic hybrid monolithic column.},
author = {Sisem Ektirici and Ilgım Göktürk and Fatma Yılmaz and A. Deni̇zli̇},
journal = {Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
doi = {10.1016/j.jchromb.2020.122477},
year = {2020}
}