Surface Plasmon Resonance-Based Immunosensor for Igm Detection with Gold Nanoparticles
Nilay Bereli̇, Monireh Bakhshpour, A. A. Topçu, A. Deni̇zli̇
- Authors
- Nilay Bereli̇, Monireh Bakhshpour, A. A. Topçu, A. Deni̇zli̇
- Published
- Micromachines 2021
- DOI
- 10.3390/mi12091092
- Citations
- 16
Abstract
In this work, a surface plasmon resonance (SPR) based immunosensor was prepared by the immobilization of the amine-functionalized gold nanoparticles (N-AuNPs) on the sensing surface to sense immunoglobulin M (IgM) antibodies in the aqueous solution and artificial plasma. The characterization studies of SPR based immunosensor for IgM detection were performed with scanning electron microscope (SEM), contact angle measurements, and ellipsometry. Kinetic studies for the IgM immunosensor were carried out in the range of 1.0 to 200 ng/mL IgM concentrations in an aqueous solution. The total IgM analysis time including adsorption, desorption, and regeneration cycles was nearly 10 min for the prepared immunosensor. The limit of detection (LOD) and limit of quantification (LOQ) were found as 0.08 and 0.26 ng/mL, respectively. The reusability of the proposed immunosensor was tested with 6 consecutive adsorption-desorption, and regeneration cycles. Also, enzyme-linked immunosorbent assay (ELISA) method was utilized in the validation of the immunosensor.
Cite this article
Nilay Bereli̇, Monireh Bakhshpour, A. A. Topçu, A. Deni̇zli̇ (2021). Surface Plasmon Resonance-Based Immunosensor for Igm Detection with Gold Nanoparticles. Micromachines. https://doi.org/10.3390/mi12091092
BibTeX
@article{nilay2021,
title = {Surface Plasmon Resonance-Based Immunosensor for Igm Detection with Gold Nanoparticles},
author = {Nilay Bereli̇ and Monireh Bakhshpour and A. A. Topçu and A. Deni̇zli̇},
journal = {Micromachines},
doi = {10.3390/mi12091092},
year = {2021}
}