Synthesis of a specific monolithic column with artificial recognition sites for L-glutamic acid via cryo-crosslinking of imprinted nanoparticles

Authors

Ilgım Göktürk, R. Uzek, L. Uzun, A. Deni̇zli̇

Published

Artificial Cells Nanomedicine and Biotechnology 2015

DOI

10.3109/21691401.2015.1011806

Citations

10

Abstract

In this study, a new molecular imprinting (MIP)-based monolithic cryogel column was prepared using chemically crosslinked molecularly imprinted nanoparticles, to achieve a simplified chromatographic separation (SPE) for a model compound, L-glutamic acid (L-Glu). Cryogelation through crosslinking of imprinted nanoparticles forms stable monolithic cryogel columns. This technique reduces the leakage of nanoparticles and increases the surface area, while protecting the structural features of the cryogel for stable and efficient recognition of the template molecule. A non-imprinted monolithic cryogel column (NIP) was also prepared, using non-imprinted nanoparticles produced without the addition of L-Glu during polymerization. The molecularly imprinted monolithic cryogel column (MIP) indicates apparent recognition selectivity and a good adsorption capacity compared to the NIP. Also, we have achieved a significant increase in the adsorption capacity, using the advantage of high surface area of the nanoparticles.

Related research

BiosensorsBiochromatographyNanomaterials

Cite this article

BibTeX

@article{ilgm2015,
  title   = {Synthesis of a specific monolithic column with artificial recognition sites for L-glutamic acid via cryo-crosslinking of imprinted nanoparticles},
  author  = {Ilgım Göktürk and R. Uzek and L. Uzun and A. Deni̇zli̇},
  journal = {Artificial Cells Nanomedicine and Biotechnology},
  doi     = {10.3109/21691401.2015.1011806},
  year    = {2015}
}

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